Tusla has announced its decision to close four Hyde & Seek creches.
The facilities cater for children from three months up to 12 years of age.
They are located at Tolka Road, Shaw Street, Millbourne Avenue, and Glasnevin.
The decision was reached after the Hyde & Seek chain featured in an RTÉ Investigates documentary earlier this year.
While researchers witnessed some examples of good care, the undercover workers started to observe repeated breaches of regulation.
RTÉ also claimed the Hyde & Seek crèche in Glasnevin went unregistered for some 14 months.
Director of Quality Assurance at Tusla, Brian Lee, said it is the agency's role is to monitor the safety and quality of care and support of children in early years’ provision.
He said, "Where there are consistent and serious breaches, Tusla will take action up to and including prosecution and removal from the register of early years’ services."
Tusla has also now committed to publicly detailing its decisions to remove services from the register in the future.
The child and family agency says the creches have been removed from the early years' services register and will close for good on 31st December this year.
Decision to Appeal
Hyde & Seek has said it will appeal the decision.
In a statement, the company said it has worked to ensure the highest standards at the facilities since the RTÉ programme aired.
It says it has confidence in the service it provides at all four creches.