Midfielder Tyler Toland has been ruled out of the Republic of Ireland's World Cup qualifiers against Slovakia and the Netherlands.
The 16-year old made her international debut in the 2-0 win over Northern Ireland at the start of the campaign after stepping up from the under-17s team.
Toland went on to start in the 2-0 win over Slovakia and the 0-0 draw against the Netherlands but will now miss the next two qualifiers with a fractured wrist.
"It's another huge blow for us as Tyler has been an influential member of the team since she made her debut at the start of the campaign," said Irish head coach Colin Bell.
"Her performances against Slovakia and the Netherlands were fantastic but now we've got to make plans for these two fixtures without her. It will give someone else in the squad an opportunity to step up and we've got to look forward now.
"The players have trained brilliantly all week and they're ready for another tough test. The whole squad are incredibly excited to play in front of our home crowd for the first time this campaign and we've got to direct that energy into a positive result."
Shelbourne full-back Niamh Prior was called up to the squad earlier in the week to replace Peamount United defender Claire Walsh who has been ruled out through illness.
Stephanie Roche, Megan Campbell, Harriet Scott and Heather Payne are already out through injury but Bell has great faith in the quality of his squad.
"We've been challenged at every stage of the campaign. We had Aine and Steph injured during the first game of the campaign and then Megan was injured after the Slovakia win.
"Add Harriet, Heather, Claire and Tyler to that list and we're missing a number of players but I believe in the quality throughout my squad and they've proven they can adjust during the campaign.
"It is just another challenge for us to get through but the players have to take on that responsibility. We've got a lot of quality in the squad and I'm sure whoever comes into the team and represents their country tomorrow will be up for the challenge."
Republic of Ireland WNT Squad
Goalkeepers: Marie Hourihan (Manchester City), Amanda Budden (Cork City), Amanda McQuillan (Shelbourne)
Defenders: Sophie Perry-Campbell (Brighton and Hove Albion), Diane Caldwell (SC Sand), Louise Quinn (Arsenal), Niamh Fahey (Bordeaux), Niamh Prior (Shelbourne).
Midfielders: Megan Connolly (Florida State University), Karen Duggan (Peamount United), Denise O'Sullivan (NC Courage), Roma McLaughlin (Shelbourne), Amy Boyle-Carr (Sion Swifts).
Attackers: Katie McCabe (Arsenal), Leanne Kiernan (Shelbourne), Amber Barrett (Peamount United), Isibeal Atkinson (Shelbourne), Ruesha Littlejohn (Celtic), Aine O'Gorman (Peamount United), Claire O'Riordan (Wexford Youths), Dearbhaile Beirne (UCD Waves)
FIFA 2019 World Cup Qualifying Group Three - Fixtures
April 6: Republic of Ireland v Slovakia, Tallaght Stadium, KO 5pm
April 10: Republic of Ireland v The Netherlands, Tallaght Stadium, KO 7pm