Atletico Madrid and Inter Milan are the latest clubs to withdraw from the proposed European Super League.
All six Premier League clubs pulled out yesterday, leaving just six European clubs remaining in the breakaway league.
The founder of the proposed project and Juventus chairman Andrea Agnelli said on Wednesday morning that the league can no longer go ahead after the six English clubs withdrew.
It did not take long for Atletico and Inter Milan to confirm that neither would be part of the league.
Atletico's statement read: "Atlético Madrid's Board of Directors, which met on Wednesday morning, has decided to formally communicate to the Super League and the rest of the founding clubs its decision not to finally formalise its adhesion to the project.
"Atlético took the decision on Monday to join the project due to circumstances that no longer exist today.
"For the club, harmony between all the groups that make up the Red & White family, especially our fans, is essential.
"The first-team squad and the coach have expressed their satisfaction with the club's decision, as they understand that sporting merits must prevail over any other criteria."
A statement from Serie A leaders Inter Milan followed shortly afterward.
"Inter Milan confirms that the Club is no longer part of the Super League project.
"We are always committed to giving the fans the best football experience; innovation and inclusion have been part of our DNA since our foundation.
"Our commitment with all stakeholders to improve the football industry will never change.
"Inter believe that football, like any sector of activity, should have an interest in constantly improving its competitions, in order to continue to excite fans of all ages all over the world, within a framework of financial sustainability.
"With this vision we will continue to work together with institutions and all stakeholders for the future of the sport we all love."
As things stand there are only four clubs yet to confirm that they have withdrawn from the league - Real Madrid, Barcelona, Juventus and AC Milan.