Transport Minister Shane Ross is accusing Danny Healy-Rae of acting like a ‘road traffic terrorist’.
The Independent Kerry TD is one of several vocal opponents in the Dáil to proposed new drink driving laws.
If passed, the bill would increase penalties for those caught driving under the influence.
It would include an automatic driving ban for first-time offenders detected at the lowest limit - a blood alcohol level of between 50mg and 80mg.
Deputy Healy-Rae last week insisted "a pint and a half never made anybody drunk".
With the Dáil set to debate the bill again later today, Shane Ross is calling on those opposing the legislation to support it.
Minister Ross argued: "I'll take this opportunity to Danny Healy-Rae and his gang to stop the filibuster.
"They're behaving like road traffic terrorists. There's a kind of guerrilla warfare going on, which is costing lives.
"I appeal to those very, very few people left opposing this in the Dáil to stop the filibuster, to stop the guerrilla warfare, and to let this legislation through which is the will of the Dáil," he added.