More than 100,000 live animals have been used for testing in Trinity College since 2012.
Last year alone, the university spent over €310,000 obtaining the animals for use in experiments and a further €10,000 disposing of the carcasses.
The Irish Independent report that rats, mice, pigs and rabbits have all been used for research purposes at Trinity over the last five years. The number of the animals used in experiments during 2016 increased by four times the amount used in 2015.
The documents, released under the Freedom of Information Act, show 109.531 live animals were bought for biomedical research by Trinity College between 2012 and 2016.
Current laws permit animal-based testing only in cases where there is no alternative method of experimentation.